| # | Eastern Conference | MP | W | D | L | G | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1. | Louisville City | 26 | 19 | 6 | 1 | 52:19 | 33 | 63 | |
| 2. | Charleston | 26 | 17 | 4 | 5 | 54:29 | 25 | 55 | |
| 3. | North Carolina | 25 | 12 | 4 | 9 | 38:34 | 4 | 40 | |
| 4. | Loudoun | 26 | 11 | 6 | 9 | 43:44 | -1 | 39 | |
| 5. | Hartford Athletic | 26 | 11 | 5 | 10 | 40:29 | 11 | 38 | |
| 6. | Pittsburgh | 26 | 10 | 7 | 9 | 26:24 | 2 | 37 | |
| 7. | Indy Eleven | 26 | 9 | 5 | 12 | 40:45 | -5 | 32 | |
| 8. | Detroit | 26 | 8 | 8 | 10 | 29:32 | -3 | 32 | |
| 9. | Rhode Island | 26 | 8 | 7 | 11 | 20:26 | -6 | 31 | |
| 10. | Tampa Bay | 26 | 7 | 6 | 13 | 35:42 | -7 | 27 | |
| 11. | Birmingham | 26 | 4 | 11 | 11 | 31:44 | -13 | 23 | |
| 12. | Miami FC | 26 | 5 | 6 | 15 | 22:40 | -18 | 21 | |
| # | Western Conference | MP | W | D | L | G | GD | Pts | Form |
| 1. | FC Tulsa | 26 | 13 | 8 | 5 | 41:29 | 12 | 47 | |
| 2. | Sacramento Republic | 25 | 11 | 8 | 6 | 37:20 | 17 | 41 | |
| 3. | New Mexico | 26 | 12 | 4 | 10 | 36:34 | 2 | 40 | |
| 4. | San Antonio | 25 | 10 | 7 | 8 | 34:31 | 3 | 37 | |
| 5. | Lexington | 26 | 9 | 8 | 9 | 28:32 | -4 | 35 | |
| 6. | El Paso | 25 | 8 | 9 | 8 | 39:37 | 2 | 33 | |
| 7. | Phoenix Rising | 26 | 7 | 12 | 7 | 45:47 | -2 | 33 | |
| 8. | Colorado Springs | 26 | 8 | 7 | 11 | 33:39 | -6 | 31 | |
| 9. | Orange County SC | 24 | 8 | 6 | 10 | 37:39 | -2 | 30 | |
| 10. | Monterey Bay | 27 | 7 | 7 | 13 | 26:37 | -11 | 28 | |
| 11. | Oakland Roots | 25 | 7 | 6 | 12 | 32:41 | -9 | 27 | |
| 12. | Las Vegas Lights | 26 | 6 | 7 | 13 | 19:43 | -24 | 25 |