| # | Eastern Conference | MP | W | D | L | G | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1. | Charleston | 12 | 10 | 1 | 1 | 32:12 | 20 | 31 | |
| 2. | Louisville City | 12 | 9 | 3 | 0 | 26:8 | 18 | 30 | |
| 3. | North Carolina | 13 | 8 | 2 | 3 | 24:17 | 7 | 26 | |
| 4. | Loudoun | 13 | 7 | 3 | 3 | 26:21 | 5 | 24 | |
| 5. | Pittsburgh | 13 | 7 | 3 | 3 | 16:11 | 5 | 24 | |
| 6. | Indy Eleven | 14 | 6 | 3 | 5 | 25:25 | 0 | 21 | |
| 7. | Detroit | 13 | 5 | 4 | 4 | 16:11 | 5 | 19 | |
| 8. | Hartford Athletic | 14 | 5 | 3 | 6 | 18:12 | 6 | 18 | |
| 9. | Rhode Island | 13 | 4 | 5 | 4 | 7:8 | -1 | 17 | |
| 10. | Tampa Bay | 12 | 3 | 4 | 5 | 18:23 | -5 | 13 | |
| 11. | Miami FC | 14 | 3 | 3 | 8 | 13:21 | -8 | 12 | |
| 12. | Birmingham | 13 | 2 | 4 | 7 | 10:19 | -9 | 10 | |
| # | Western Conference | MP | W | D | L | G | GD | Pts | Form |
| 1. | FC Tulsa | 12 | 7 | 3 | 2 | 21:14 | 7 | 24 | |
| 2. | Lexington | 13 | 6 | 5 | 2 | 17:12 | 5 | 23 | |
| 3. | Orange County SC | 11 | 6 | 3 | 2 | 27:20 | 7 | 21 | |
| 4. | Monterey Bay | 13 | 6 | 3 | 4 | 18:19 | -1 | 21 | |
| 5. | New Mexico | 12 | 6 | 2 | 4 | 18:13 | 5 | 20 | |
| 6. | San Antonio | 14 | 6 | 2 | 6 | 18:15 | 3 | 20 | |
| 7. | El Paso | 13 | 5 | 4 | 4 | 27:21 | 6 | 19 | |
| 8. | Sacramento Republic | 12 | 4 | 7 | 1 | 20:11 | 9 | 19 | |
| 9. | Las Vegas Lights | 14 | 5 | 3 | 6 | 12:15 | -3 | 18 | |
| 10. | Colorado Springs | 13 | 5 | 2 | 6 | 13:14 | -1 | 17 | |
| 11. | Phoenix Rising | 13 | 3 | 7 | 3 | 26:22 | 4 | 16 | |
| 12. | Oakland Roots | 13 | 3 | 3 | 7 | 9:16 | -7 | 12 |